Dominant clones in immortalized T‐cell lines from rheumatoid arthritis synovial membranes
Identifieur interne : 000312 ( France/Analysis ); précédent : 000311; suivant : 000313Dominant clones in immortalized T‐cell lines from rheumatoid arthritis synovial membranes
Auteurs : A. M. Saadawi ; F. L'Faqihi ; B. Y. Diab ; M. A. Sol ; G. Enault ; H. Coppin ; A. Cantagrel [France] ; B. Biesinger [Allemagne] ; B. Fleckenstein [Allemagne] ; M. Thomsen [France]Source :
- Tissue Antigens [ 0001-2815 ] ; 1997-05.
English descriptors
- Teeft :
- Accessory cells, Allogeneic feeder cells, Antigen specificity, Arthritis, Arthritis rheum, Cdr3 region, Cell line, Cell receptor, Clone, Cord blood, Dominant clones, Feeder cells, First stimulation, Fleckenstein, Herpesvirus, Herpesvirus saimiri, Hybridization, Hybridization medium, Hybridization signal, Infectious virus, Lymphocyte, Nontransformed cells, Other lines, Peripheral blood, Previous studies, Primer, Receptor, Rheumatoid, Rheumatoid arthritis, Rheumatoid arthritis synovial membranes, Rheumatoid lines, Rheumatoid synovium, Saimiri, Several lines, Sitir hybridization, Specific antigen, Squirrel monkey, Successful transformation, Surface markers, Synovial, Synovial membrane, Synovial membranes, Synovial tissue, Tcrbv genes, Viral.
Abstract
Transformation of human T cells by herpesvirus saimiri allows the production of an unlimited number of T cells which express a functional T‐cell receptor. In this study we transformed four T‐cell lines derived from rheumatoid arthritis synovial membranes. The transformed T cells were mainly CD4+ and expressed the phenotype of activated T cells. They were grown for more than 1 year in the absence of mitogen or feeder cells, and three of them could be maintained without exogenous LL‐2. The presence of viral DNA in the transformed cells was shown by in situ hybridization with a probe from the H‐DNA region of the virus. No infectious virus could be recovered from the transformed cells. The relative proportion of the 24 different Vβ families between the four transformed lines showed variations that increased with time. In the two T‐cell lines transformed at an early stage of culture, the Vβ2 family was maintained at about 10%. The dominant Vβ2 clones that previously have been characterized in the patient were found in all transformed T‐cell lines. We have thus shown the feasibility of obtaining transformed T cells from synovia] membranes. They contain the dominant clones that are considered of potential importance for the disease, permitting further functional studies.
Url:
DOI: 10.1111/j.1399-0039.1997.tb02775.x
Affiliations:
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Biesinger</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Klinische und Molekuläre Virologie der Universität Eriangen‐Nürnberg</wicri:regionArea></affiliation></author><author><name sortKey="Fleckenstein, B" sort="Fleckenstein, B" uniqKey="Fleckenstein B" first="B." last="Fleckenstein">B. Fleckenstein</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Klinische und Molekuläre Virologie der Universität Eriangen‐Nürnberg</wicri:regionArea></affiliation></author><author><name sortKey="Thomsen, M" sort="Thomsen, M" uniqKey="Thomsen M" first="M." last="Thomsen">M. Thomsen</name><affiliation></affiliation><affiliation wicri:level="1"><country wicri:rule="url">France</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Tissue Antigens</title><title level="j" type="alt">TISSUE ANTIGENS</title><idno type="ISSN">0001-2815</idno><idno type="eISSN">1399-0039</idno><imprint><biblScope unit="vol">49</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="431">431</biblScope><biblScope unit="page" to="437">437</biblScope><biblScope unit="page-count">7</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1997-05">1997-05</date></imprint><idno type="ISSN">0001-2815</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0001-2815</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Accessory cells</term><term>Allogeneic feeder cells</term><term>Antigen specificity</term><term>Arthritis</term><term>Arthritis rheum</term><term>Cdr3 region</term><term>Cell line</term><term>Cell receptor</term><term>Clone</term><term>Cord blood</term><term>Dominant clones</term><term>Feeder cells</term><term>First stimulation</term><term>Fleckenstein</term><term>Herpesvirus</term><term>Herpesvirus saimiri</term><term>Hybridization</term><term>Hybridization medium</term><term>Hybridization signal</term><term>Infectious virus</term><term>Lymphocyte</term><term>Nontransformed cells</term><term>Other lines</term><term>Peripheral blood</term><term>Previous studies</term><term>Primer</term><term>Receptor</term><term>Rheumatoid</term><term>Rheumatoid arthritis</term><term>Rheumatoid arthritis synovial membranes</term><term>Rheumatoid lines</term><term>Rheumatoid synovium</term><term>Saimiri</term><term>Several lines</term><term>Sitir hybridization</term><term>Specific antigen</term><term>Squirrel monkey</term><term>Successful transformation</term><term>Surface markers</term><term>Synovial</term><term>Synovial membrane</term><term>Synovial membranes</term><term>Synovial tissue</term><term>Tcrbv genes</term><term>Viral</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Transformation of human T cells by herpesvirus saimiri allows the production of an unlimited number of T cells which express a functional T‐cell receptor. In this study we transformed four T‐cell lines derived from rheumatoid arthritis synovial membranes. The transformed T cells were mainly CD4+ and expressed the phenotype of activated T cells. They were grown for more than 1 year in the absence of mitogen or feeder cells, and three of them could be maintained without exogenous LL‐2. The presence of viral DNA in the transformed cells was shown by in situ hybridization with a probe from the H‐DNA region of the virus. No infectious virus could be recovered from the transformed cells. The relative proportion of the 24 different Vβ families between the four transformed lines showed variations that increased with time. In the two T‐cell lines transformed at an early stage of culture, the Vβ2 family was maintained at about 10%. The dominant Vβ2 clones that previously have been characterized in the patient were found in all transformed T‐cell lines. We have thus shown the feasibility of obtaining transformed T cells from synovia] membranes. They contain the dominant clones that are considered of potential importance for the disease, permitting further functional studies.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>France</li></country><region><li>Midi-Pyrénées</li><li>Occitanie (région administrative)</li></region><settlement><li>Toulouse</li></settlement></list><tree><noCountry><name sortKey="Coppin, H" sort="Coppin, H" uniqKey="Coppin H" first="H." last="Coppin">H. Coppin</name><name sortKey="Diab, B Y" sort="Diab, B Y" uniqKey="Diab B" first="B. Y." last="Diab">B. Y. Diab</name><name sortKey="Enault, G" sort="Enault, G" uniqKey="Enault G" first="G." last="Enault">G. Enault</name><name sortKey="L Faqihi, F" sort="L Faqihi, F" uniqKey="L Faqihi F" first="F." last="L'Faqihi">F. L'Faqihi</name><name sortKey="Saadawi, A M" sort="Saadawi, A M" uniqKey="Saadawi A" first="A. M." last="Saadawi">A. M. Saadawi</name><name sortKey="Sol, M A" sort="Sol, M A" uniqKey="Sol M" first="M. A." last="Sol">M. A. Sol</name></noCountry><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Cantagrel, A" sort="Cantagrel, A" uniqKey="Cantagrel A" first="A." last="Cantagrel">A. Cantagrel</name></region><name sortKey="Thomsen, M" sort="Thomsen, M" uniqKey="Thomsen M" first="M." last="Thomsen">M. Thomsen</name></country><country name="Allemagne"><noRegion><name sortKey="Biesinger, B" sort="Biesinger, B" uniqKey="Biesinger B" first="B." last="Biesinger">B. Biesinger</name></noRegion><name sortKey="Fleckenstein, B" sort="Fleckenstein, B" uniqKey="Fleckenstein B" first="B." last="Fleckenstein">B. Fleckenstein</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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